First off, don't just remove the wheel because you think it will help. You need a guide pin to replace that wheel, that car could go flying when it gets to the stop sections, thats if you have the tune right and it don't fly off the track before the end. /images/boards/smilies/wink.gif There is a reason why guys use a guide pin.
So, you ask if you it will affect your tune. I'd say yes, because that 2 grams was in a different location. If you really have that car dialed with the 4th wheel in place you have a chance that it will change the tune. So if you want to run 3 wheels then tune the car that why in the first place. This way you can figure out where to put that 2 grams somewhere else. /images/boards/smilies/wink.gif